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recombinant human plk3 protein #pr7316b  (Thermo Fisher)


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    Thermo Fisher recombinant human plk3 protein #pr7316b
    The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and <t>PLK3-transfected</t> HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.
    Recombinant Human Plk3 Protein #Pr7316b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+plk3+protein/pmc11549080-222-9-12?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    recombinant human plk3 protein #pr7316b - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain"

    Article Title: Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain

    Journal: NPJ Parkinson's Disease

    doi: 10.1038/s41531-024-00830-y

    The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and PLK3-transfected HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.
    Figure Legend Snippet: The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and PLK3-transfected HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.

    Techniques Used: Transfection, Inter Assay, Construct, Knock-Out, Serial Dilution, Standard Deviation



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    recombinant human plk3 protein #pr7316b  (Thermo Fisher)
    90
    Thermo Fisher recombinant human plk3 protein #pr7316b
    The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and <t>PLK3-transfected</t> HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.
    Recombinant Human Plk3 Protein #Pr7316b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+plk3+protein/pmc11549080-222-9-12?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    recombinant human plk3 protein #pr7316b - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    recombinant human plk3 protein  (Thermo Fisher)
    90
    Thermo Fisher recombinant human plk3 protein
    The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and <t>PLK3-transfected</t> HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.
    Recombinant Human Plk3 Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+plk3+protein/pm39516469-200-8-10?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    recombinant human plk3 protein - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and PLK3-transfected HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.

    Journal: NPJ Parkinson's Disease

    Article Title: Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain

    doi: 10.1038/s41531-024-00830-y

    Figure Lengend Snippet: The most sensitive new pS129 α-synuclein assay (left) was characterized using wild-type (WT) mouse brain tissue extracts and PLK3-transfected HEK293 cell lysates ( a , b ), while testing of the most sensitive total α-synuclein assay (right) utilized WT mouse brain tissue extracts and WT HEK293 cell lysates ( c , d ). Extracts and lysates were serially diluted using assay buffer. Associated assay data for all formulations tested are contained in Supplementary Table . Inter-assay variation (RSD) data presented in ( b ) and ( d ) were calculated from three individual dilution curves constructed and measured on separate days, with data only shown for extract/lysate dilutions above the lower limit of quantification (LLoQ). Phospho-S129 ( e , f ) and total ( g , h ) assay specificity were also assessed using SNCA knock-out (KO) HEK293 cell lysates and Snca KO mouse brain tissue extracts. Protein concentrations in each sample prior to serial dilution were as follows: WT mouse brain supernatant, 4.93 mg/mL; KO mouse brain supernatant, 5.48 mg/mL; WT HEK293 supernatant, 4.77 mg/mL; KO HEK293 supernatant, 5.6 mg/mL; PLK3-HEK293 supernatant, 6.55 mg/mL. The limit of detection (LoD) and LLoQ were defined as described in Fig. . Data in ( a , b , g , h ) represent mean ± standard deviation. For all standard curves; number of independent experiments ( N ) = 3, number of replicates in each experiment ( n = 3). AU arbitrary units.

    Article Snippet: Phosphorylation was induced by incubating 1 μg of recombinant human PLK3 protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 μL of mouse WT α-synuclein in kinase reaction buffer for 8 h at 30 °C.

    Techniques: Transfection, Inter Assay, Construct, Knock-Out, Serial Dilution, Standard Deviation